Part:BBa_K258006:Design
Thermostable lipase (TliA) of Pseudomonas fluorescens SIK W1
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 651
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence has being optimized for Biobrick restriction sites
Source
The thermostable lipase (TliA) of P. fluorescens SIK W1 is comprised of 476 amino acids and has the characteristic C-terminal signal sequence recognized by the ABC transporter.
References
[1]Export of recombinant proteins in Escherichia coli using ABC
transporter with an attached lipase ABC transporter recognition
domain (LARD)
Chan Woo Chung1, Jinsun You1, Kyeongyeon Kim1, Yuseok Moon2,
Hoeon Kim3 and Jung Hoon AhnPublished: 29 January 2009
Microbial Cell Factories 2009, 8:11 doi:10.1186/1475-2859-8-11
Received: 18 November 2008
Accepted: 29 January 2009
This article is available from: http://www.microbialcellfactories.com/content/8/1/11
[2]Ahn JH, Pan JG, Rhee JS: Identification of the tliDEF ABC transporter
specific for lipase in Pseudomonas fluorescens SIK W1.
J Bacteriol 1999, 181(6):1847-1852.
[3]Guzzo J, Duong F, Wandersman C, Murgier M, Lazdunski A: The
secretion genes of Pseudomonas aeruginosa alkaline protease
are functionally related to those of Erwinia chrysanthemi proteases
and Escherichia coli alpha-haemolysin. Mol Microbiol
1991, 5(2):447-453.